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superscript iii first-strand synthesis supermix  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript iii first-strand synthesis supermix
    Superscript Iii First Strand Synthesis Supermix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iii+first-strand+synthesis+supermix/superscript+iii+first+strand+synthesis+supermix/pmc12263051-120-7-12
    Average 90 stars, based on 1 article reviews
    superscript iii first-strand synthesis supermix - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: PCOS endometrium-derived epithelial organoids as a novel model to study endometrial dysfunction.
    Article Snippet: .. For reverse-transcription quantitative PCR (RT-qPCR), cDNA was synthesized using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific) or Superscript III First-Strand Synthesis SuperMix (ThermoFisher Scientific) followed by qPCR using triplicate cDNA samples on the StepOneTM Real-Time PCR system (Applied Biosystems, USA). ..

    Article Title: CRISPR-mediated proximity labeling unveils ABHD14B as a host factor to regulate HBV cccDNA transcriptional activity
    Article Snippet: Total RNA was extracted from the tissues using RNeasy with DNase treatment (Qiagen). .. The extracted RNA was reverse transcribed using SuperScript III First-Strand Synthesis SuperMix (Thermo Fisher Scientific). .. Quantitative PCR was carried out using Thunderbird SYBR qPCR Mix (Toyobo) and the StepOnePlus Real-Time PCR System (Life Technologies).

    Article Title: In vivo AGO-APP identifies a module of microRNAs cooperatively preserving neural progenitors.
    Article Snippet: After centrifugation the EtOH-precipitated fragments were resuspended in water and ligated to 0.25 μ M randomized 5’ RNA adapter using T4 RNA ligase 1 (NEB) in the same buffer conditions as described above. .. Reverse transcription with Superscript III First-Strand synthesis supermix (ThermoFisher Scientific) and 5 μ M RT Primer (RTP, TruSeq kit; Illumina) was then performed according to the manufacturer’s recommendations. ..

    Real-time Polymerase Chain Reaction:

    Article Title: PCOS endometrium-derived epithelial organoids as a novel model to study endometrial dysfunction.
    Article Snippet: .. For reverse-transcription quantitative PCR (RT-qPCR), cDNA was synthesized using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific) or Superscript III First-Strand Synthesis SuperMix (ThermoFisher Scientific) followed by qPCR using triplicate cDNA samples on the StepOneTM Real-Time PCR system (Applied Biosystems, USA). ..

    Article Title: The Effects of Wuniuzao Green Tea on Mice With High‐Fat Diet‐Induced Liver Steatosis
    Article Snippet: The Nanodrop 2000 UV–Vis Spectrophotometer (Beckman, USA) was then used to spectrophotometrically measure the RNA quantity. .. SuperScript III First‐Strand Synthesis SuperMix (Thermo Fisher, USA) was used to create single‐stranded cDNA from total RNA, and Power SYBR Green PCR Master Mix was used to conduct real‐time PCR (Applied Biosystems). ..

    Synthesized:

    Article Title: PCOS endometrium-derived epithelial organoids as a novel model to study endometrial dysfunction.
    Article Snippet: .. For reverse-transcription quantitative PCR (RT-qPCR), cDNA was synthesized using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific) or Superscript III First-Strand Synthesis SuperMix (ThermoFisher Scientific) followed by qPCR using triplicate cDNA samples on the StepOneTM Real-Time PCR system (Applied Biosystems, USA). ..

    Article Title: Nicotiana benthamiana VASCULAR-RELATED NAC-DOMAIN7-2 ( NbVND7-2 ) has a role in xylem formation during interfamily grafting
    Article Snippet: .. Total RNA was isolated from N. benthamiana plants using the RNeasy Plant Mini Kit (Qiagen). cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Sex differences in placental structure and gene expression in ICR mice during embryonic development.
    Article Snippet: Prior to the synthesis of single-stranded cDNA, genomic DNA contamination was removed from the total RNA using DNase I (#18068015; Thermo Fisher Scientific, Waltham, MA, USA). .. Single-stranded cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (#18080400; Thermo Fisher Scientific) and subsequently amplified by PCR. qRT-PCR was performed with PowerUp SYBR Green Master Mix (#A25742; Thermo Fisher Scientific) on a QuantStudio 7 Flex system under cycling conditions of 50 °C for 2 min, 95 °C for 2 min, Scientific Reports | (2025) 15:24067 6| https://doi.org/10.1038/s41598-025-10476-2 followed by 40 cycles of 95 °C for 1 sec and 60 °C for 30 sec. Each biological sample had four technical replicates, normalized to 18S rRNA. ..

    cDNA Synthesis:

    Article Title: PCOS endometrium-derived epithelial organoids as a novel model to study endometrial dysfunction.
    Article Snippet: .. For reverse-transcription quantitative PCR (RT-qPCR), cDNA was synthesized using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific) or Superscript III First-Strand Synthesis SuperMix (ThermoFisher Scientific) followed by qPCR using triplicate cDNA samples on the StepOneTM Real-Time PCR system (Applied Biosystems, USA). ..

    Isolation:

    Article Title: Nicotiana benthamiana VASCULAR-RELATED NAC-DOMAIN7-2 ( NbVND7-2 ) has a role in xylem formation during interfamily grafting
    Article Snippet: .. Total RNA was isolated from N. benthamiana plants using the RNeasy Plant Mini Kit (Qiagen). cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Amplification:

    Article Title: Sex differences in placental structure and gene expression in ICR mice during embryonic development.
    Article Snippet: Prior to the synthesis of single-stranded cDNA, genomic DNA contamination was removed from the total RNA using DNase I (#18068015; Thermo Fisher Scientific, Waltham, MA, USA). .. Single-stranded cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (#18080400; Thermo Fisher Scientific) and subsequently amplified by PCR. qRT-PCR was performed with PowerUp SYBR Green Master Mix (#A25742; Thermo Fisher Scientific) on a QuantStudio 7 Flex system under cycling conditions of 50 °C for 2 min, 95 °C for 2 min, Scientific Reports | (2025) 15:24067 6| https://doi.org/10.1038/s41598-025-10476-2 followed by 40 cycles of 95 °C for 1 sec and 60 °C for 30 sec. Each biological sample had four technical replicates, normalized to 18S rRNA. ..

    Polymerase Chain Reaction:

    Article Title: Sex differences in placental structure and gene expression in ICR mice during embryonic development.
    Article Snippet: Prior to the synthesis of single-stranded cDNA, genomic DNA contamination was removed from the total RNA using DNase I (#18068015; Thermo Fisher Scientific, Waltham, MA, USA). .. Single-stranded cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (#18080400; Thermo Fisher Scientific) and subsequently amplified by PCR. qRT-PCR was performed with PowerUp SYBR Green Master Mix (#A25742; Thermo Fisher Scientific) on a QuantStudio 7 Flex system under cycling conditions of 50 °C for 2 min, 95 °C for 2 min, Scientific Reports | (2025) 15:24067 6| https://doi.org/10.1038/s41598-025-10476-2 followed by 40 cycles of 95 °C for 1 sec and 60 °C for 30 sec. Each biological sample had four technical replicates, normalized to 18S rRNA. ..

    Article Title: The Effects of Wuniuzao Green Tea on Mice With High‐Fat Diet‐Induced Liver Steatosis
    Article Snippet: The Nanodrop 2000 UV–Vis Spectrophotometer (Beckman, USA) was then used to spectrophotometrically measure the RNA quantity. .. SuperScript III First‐Strand Synthesis SuperMix (Thermo Fisher, USA) was used to create single‐stranded cDNA from total RNA, and Power SYBR Green PCR Master Mix was used to conduct real‐time PCR (Applied Biosystems). ..

    Quantitative RT-PCR:

    Article Title: Sex differences in placental structure and gene expression in ICR mice during embryonic development.
    Article Snippet: Prior to the synthesis of single-stranded cDNA, genomic DNA contamination was removed from the total RNA using DNase I (#18068015; Thermo Fisher Scientific, Waltham, MA, USA). .. Single-stranded cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (#18080400; Thermo Fisher Scientific) and subsequently amplified by PCR. qRT-PCR was performed with PowerUp SYBR Green Master Mix (#A25742; Thermo Fisher Scientific) on a QuantStudio 7 Flex system under cycling conditions of 50 °C for 2 min, 95 °C for 2 min, Scientific Reports | (2025) 15:24067 6| https://doi.org/10.1038/s41598-025-10476-2 followed by 40 cycles of 95 °C for 1 sec and 60 °C for 30 sec. Each biological sample had four technical replicates, normalized to 18S rRNA. ..

    SYBR Green Assay:

    Article Title: Sex differences in placental structure and gene expression in ICR mice during embryonic development.
    Article Snippet: Prior to the synthesis of single-stranded cDNA, genomic DNA contamination was removed from the total RNA using DNase I (#18068015; Thermo Fisher Scientific, Waltham, MA, USA). .. Single-stranded cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (#18080400; Thermo Fisher Scientific) and subsequently amplified by PCR. qRT-PCR was performed with PowerUp SYBR Green Master Mix (#A25742; Thermo Fisher Scientific) on a QuantStudio 7 Flex system under cycling conditions of 50 °C for 2 min, 95 °C for 2 min, Scientific Reports | (2025) 15:24067 6| https://doi.org/10.1038/s41598-025-10476-2 followed by 40 cycles of 95 °C for 1 sec and 60 °C for 30 sec. Each biological sample had four technical replicates, normalized to 18S rRNA. ..

    Article Title: The Effects of Wuniuzao Green Tea on Mice With High‐Fat Diet‐Induced Liver Steatosis
    Article Snippet: The Nanodrop 2000 UV–Vis Spectrophotometer (Beckman, USA) was then used to spectrophotometrically measure the RNA quantity. .. SuperScript III First‐Strand Synthesis SuperMix (Thermo Fisher, USA) was used to create single‐stranded cDNA from total RNA, and Power SYBR Green PCR Master Mix was used to conduct real‐time PCR (Applied Biosystems). ..

    Generated:

    Article Title: Fibroblast Growth Factor 23 and Sarcopenia in Maintenance Haemodialysis Population
    Article Snippet: The RNA yield and quality were assessed using a NanoDrop Lite spectrophotometer (Thermo Fisher Scientific). .. Complementary DNA (cDNA) was generated using the Superscript III First‐Strand Synthesis SuperMix (Thermo Fisher Scientific) with a total RNA input of 150 ng. .. RT‐PCR was carried out using TaqMan Gene Expression Assays (Thermo Fisher Scientific; see for details) with the CFX Opus 384 Real‐Time PCR system (BIO‐RAD).

    other:

    Article Title: Identification of an a-factor-like pheromone secreted by the heterothallic ascomycete Aspergillus fumigatus.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DESeq2 Love et al.78 https://bioconductor.org Sequence Detection System (SDS) v2.4 Applied Biosystems N/A Other NuPAGE 4-12% Bi-Tris Gel Invitrogen Product No. NP0321 NuPAGE 1x MOPS SDS Running Buffer Invitrogen Product No. NP0001 PVDF Transfer Membrane Thermo Scientific Product No. 88518 Parafilm M sealing film Sigma-Aldrich Product No. P7793-1EA Amberlite XAD -2 Sigma-Aldrich Catalog No. 10357



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